Making a Dilution Series

Aim: to make a dilution series to investigate concentration

Equipment: Test Tube Rack, Test Tubes, 10mL measuring cylinder, a plastic transfer pipette, a potassium permanganate.

Method:

1. Fill measuring cylinder with 10mL of water
2. Add 10mL of water to a test tube
3. Add 5mL of water to 5 more tests tubes.
4. Add a small spatulaful of Potassium Permanganate to the first 10mL test tube of water
5. Add 5mL of the dark purple Potassium Permanganate into the second test tube.
6. Add 5mL of the second tube into the third test tube.
7.  Add 5mL of the third test tube into the fourth test tube. 
8. Add 5mL of the fourth test tube into the fifth test tube.
9. Add 5mL of the fifth test tube into the sixth test tube.

Results:

Discussion:

The first test tube went a dark purple colour.

As we poured 5mL from each test tube into the next test tube, the colour lightened each time.